Research Context
Glutathione is the most abundant intracellular thiol in mammalian cells and serves as the primary non-enzymatic antioxidant defense system. With over 180,000 PubMed-indexed articles, it is one of the most extensively studied molecules in biochemistry.
As a reference standard, glutathione is used in redox chemistry assays, enzymatic activity studies (glutathione peroxidase, glutathione S-transferase, glutathione reductase), oxidative stress quantification, and as a standard for thiol-specific analytical methods. Its well-characterized spectral properties and established assay protocols make it an essential reagent in any analytical chemistry bench focused on redox biology.
Key References
Meister A, Anderson ME. (1983). "Glutathione." Ann Rev Biochem. 52:711-760. PMID: 6137189
Forman HJ, et al. (2009). "Glutathione: overview of its protective roles, measurement, and biosynthesis." Mol Aspects Med. 30(1-2):1-12. PMID: 18796312
Lu SC. (2013). "Glutathione synthesis." Biochim Biophys Acta. 1830(5):3143-3153. PMID: 22995213