Research Context
NAD+ is a coenzyme found in all living cells that plays a central role in cellular metabolism, serving as an electron carrier in redox reactions and as a substrate for enzymes including sirtuins and poly(ADP-ribose) polymerases (PARPs). It is one of the most fundamental molecules in biochemistry, with over 50,000 PubMed-indexed articles.
As a reference standard, NAD+ is widely used in enzymatic assay development, sirtuin activity studies, and metabolic pathway characterization. It is also the subject of extensive research in cellular aging and metabolic regulation.
Key References
Verdin E. (2015). "NAD⁺ in aging, metabolism, and neurodegeneration." Science. 350(6265):1208-1213. PMID: 26785480
Imai S, Guarente L. (2014). "NAD+ and sirtuins in aging and disease." Trends Cell Biol. 24(8):464-471. PMID: 24786309
Yoshino J, et al. (2018). "NAD⁺ intermediates: the biology and therapeutic potential." Cell Metab. 27(3):513-528. PMID: 29249689